So, an often discussed topic on online forums is what capture settings should I use, how much should I cool my CMOS camera, what exposure, and what gain.
The answers to all these questions depend on your equipment and the amount of light pollution at your site.
The following video was given at a recent Astronomy gathering by Dr Robin Glover, which gives a real insight into how far you should take the extreme possible settings of your equipment in order to optimise the gain in quality for the amount of pain and patience needed.

Towards the end of the video Dr Robin Glover ran out of time, but later posted the missing parts with relation to what gain settings are helpful in various set ups.